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. Author manuscript; available in PMC: 2020 Sep 25.
Published in final edited form as: J Am Chem Soc. 2019 Sep 11;141(38):15250–15265. doi: 10.1021/jacs.9b07152

Figure 3.

Figure 3.

Preparation of split and supercharged GFP constructs in this study. (A) Cartoon illustration of the semisynthetic strategy to incorporate various mutations of T203 on β-strand 10 (s10). (B) Cartoon schematic depicting the insertion of a mature chromophore (mat) within the internal helix (ih) into an empty β-barrel containing the R96 mutations. This split GFP strategy enables the study of proteins for which chromophore maturation is hindered.45 See section S1 in the Supporting Information or ref 14 for an explanation of the split GFP nomenclature, logic, and details of assembly. (C) Representation of the surface charge patterns of the supercharged GFP mutants in this work.