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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2020 Jan 30;40(3):597–610. doi: 10.1161/ATVBAHA.119.313744

Figure 6.

Figure 6.

IgE reduces MSRN gene expression and promotes macrophages cholesterol loading. ORO staining and quantification of WT (A) and Fcer1α−/−Nhe1+/− (B) BMDM after 48 hrs of ox-LDL (2 mg/mL) and IFN-γ (12 ng/mL) stimulation with or without IgE (50 μg/mL). Scale: 50 μm. Representative images are shown to the left. RT-PCR analysis of MSRN genes in BMDM from WT (C), Fcer1α−/− (D), and Fcer1α−/−Nhe1+/− BMDM (E) after ox-LDL and IFN-γ stimulation with or without IgE. (F) RT-PCR analysis of MSRN genes after ox-LDL and IFN-γ stimulation with or without IgE and anti-IgE antibody in BMDM from WT mice. (G) Immunoblots detected the expression of C3, pERK, ERK, pp38 and p38 after ox-LDL and IFN-γ stimulation with or without IgE in BMDM from WT, Fcer1α−/− and Fcer1α−/−Nhe1+/− mice. RT-PCR analyses of M1 macrophage marker genes after LPS stimulation (H) and M2 macrophage marker genes after IL-4 stimulation (I) with or without IgE in BMDM from C3−/− mice. (J) Immunoblots detected the expression of pERK, ERK, pp38 and p38 after ox-LDL and IFN-γ stimulation with or without IgE in BMDM from C3−/− mice. Data are mean±SEM of four independent experiments. *P<0.05, **P<0.01, ***P<0.001.