Skip to main content
. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Clin Exp Allergy. 2020 Feb 7;50(3):401–405. doi: 10.1111/cea.13554

Figure 2.

Figure 2.

Frame A: Preparative reversed-phase (RP) chromatography of the peanut fraction purified by cation exchange and SEC. The black line indicates allergenic activity, as measured by RAST inhibition using the BPP+ reference serum. The allergenic activity is indicated on the y-axis on the right using the starting material (i.e. the peanut fraction purified by cation exchange and SEC) as 100% value. The red line indicates the optical density at 280 nm. Frames B and C: Silver-stained SDS-PAGE (B: NR=non-reduced; C: R=Reduced) of the starting material (“pre”) and the RP fractions. The green, blue and purple colored rectangles indicate our provisional assignments of the allergens mentioned in the text. Arah1Pro is the propeptide of Ara h 1. LTP-9, 16 and 17 are Ara h 9, Ara h 16 and Ara h 17; Defensin 12 and 13 are Ara h 12 and Ara h 13. The proteins indicated by the orange-colored rectangle of fractions 59–61 have not been investigated.