Table 1.

Apparent Melting Temperatures (T_m,app) of Co-assemblies Formed Between VIQKI Variants and HPIV3-HRN or RSV-HRN

	HPIV3-HRN		RSV-HRN
Inhibitor	Tm,appa (°C)	ΔTm,app†b	Tm,appa (°C)	ΔTm,app††c
VIQKI	88.6	-	64.3	-
VIQKI-V449F	-	-	41.9	−22.4
VIQKI-A450F	-	-	45.3	−19.0
VIQKI-L451F	-	-	65.9	+1.6
VIQKI-D452F	-	-	42.2	−22.1
VIQKI-P453F	-	-	63.0	−1.3
VIQKI-I454F	-	-	65.1	+0.8
VIQKI-D455F	-	-	55.6	−8.7
VIQKI-I456F	84.9	−3.7	70.0	+5.7
VIQKI-L451F/I456F	83.1	−5.5	67.2	+2.9
VIQKI-I454F/I456F	85.1	−3.5	72.8	+8.5

^a1:1 mixture of Inhibitor (50 μM) and HRN (50 μM) peptides.

^bΔT_m,app^†=T_m,app(HPIV3-HRN+Inhibitor)–T_m,app(HPIV3-HRN+VIQKI).

^cΔT_m,app^††=T_m,app(RSV-HRN+Inhibitor)–T_m,app(RSV-HRN+VIQKI).