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. Author manuscript; available in PMC: 2020 Feb 28.
Published in final edited form as: J Comp Neurol. 2016 Jul 25;527(1):159–173. doi: 10.1002/cne.24074

Figure 3.

Figure 3

Selective subpopulations of ACs are lost in experimental glaucoma. A: In control retinas there was a large number of cells in the INL and GCL that were CR-IR with dendritic processes forming characteristic three bands in the IPL. B,C,D: Eight weeks after initial bead injection there was a significant reduction in the number of CR-positive cells in both the INL and GCL as compared to values in control (Ctr) retinas. Histograms show a significant reduction in CR-positive cells beginning by 4 weeks after microbead injection. E: GABA immunoreactivity was observed in conventional ACs at the inner margin of the INL and in some dACs in the GCL of control retinas. F: The number of GABA-positive ACs and dACs was significantly reduced eight weeks after bead injection. G,H: Histograms show the time course of changes in the number of GABA-positive ACs and dACs in the INL and GCL, respectively. I,J: ChAT-immunoreactive starburst ACs were found in the GCL and proximal INL with two well defined dendritic bands in the IPL. K: There was no significant change in the number of ChAT-positive cells in the INL or GCL at eight weeks after microbead injection (p>0.5). L,M: GlyT1 immunoreactivity was present in a large number of somata in the INL and their dendritic processes within the IPL. N: There was no significant difference in the number of GlyT1-positive ACs in control retinas and in microbead-injected retinas after eight weeks (p>0.1). Bars indicate mean value ± SEM. *** p<0.001; Student’s t-test. Scale bars =50 μm for all micrographs.