Table 1.

Summary of gene expression in ovarian follicles following treatment with FSH glycoforms (100 ng/ml) and comparison to in vivo gene expression results in whole ovaries.

Gene	One-hour (in vitro)		One-hour (in vivo) 1		18-20 hours (in vitro)
	Up or down regulated relative to control	Up or down regulated between glycoforms	Up or down regulated relative to control	Up or down regulated between glycoforms	Up or down regulated relative to control	Up or down regulated between glycoforms
Apaf1	21	NS	21, 24	21 > 24	NS	NS
Casp3	24	24 > 21	21, 24	21 > 24	21	NS
Cebpb	24	24 > 21	NS	NS	NS	21 > 24
Cyp19a1	21, 24	24 > 21	21, 24	NS	21, 24	NS
Ereg	21	NS	21, 24	NS	21, 24	NS
Fshr	24	NS	21, 24	21 > 24	21, 24	NS
Inhbb	24	NS	24	NS	NS	NS
Ki67	NS	NS	21, 24	NS	24	NS
S100a6	NS	NS	NS	NS	21, 24	NS
Tagln	21	NS	21	NS	21, 24	NS

¹In vivo data from WANG, H., MAY, J., BUTNEV, V., SHUAI, B., MAY, J. V., BOUSFIELD, G. R. & KUMAR, T. R. 2016. Evaluation of in vivo bioactivities of recombinant hypo- (FSH(21/18)) and fully- (FSH(24)) glycosylated human FSH glycoforms in Fshb null mice. Mol Cell Endocrinol, 437, 224-236.

Bolded text indicates upregulation of gene expression and text not bolded denotes downregulation of gene expression. Data indicated as “NS” was not significant.