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. 2020 Feb 21;8:119. doi: 10.3389/fbioe.2020.00119

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Copyright © 2020 Yang, Xie, Wu, Liao, Chen, Hao, Wang, Zhu and Teng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The characterization of antigen-extracted mineralized collagen fibril. (A) The SEM image of bone during the process. (a) Fresh bone, bone subjected to the first methanol and chloroform process (b), hydrogen peroxide process (c), and second methanol and chloroform process (d). (B) The SEM and (C) EDX spectrum of mineralized collagen fibril. (D) The XRD spectra. (E) Representative TEM images of mineralized collagen fibril. The triangles in (a) indicated the individual mineralized collagen fibrils merged into coarse bundle, the face-on (b) and edge-on (c) view of nHA in mineralized collagen fibril. (F) The TGA result of mineralized collagen fibril following combusted to 1000°C in the air. (G) The different concentrations of extract of mineralized collagen fibril on the viability of MG63 cell (n = 4, experiments performed in triplicate. The bars show the mean ± SD).