Table 1.
Distribution of DCX positive labeling in the cerebral cortexes of adult primates with or without tissue and antigen adsorption
| Primary antibodies/disposition/brain region | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Abcam 18723-1 (Lot.GR281032-1) | Abcam 18723-2 (Lot.GR3224908-1) | ||||||||||
| Unabsorbed | Tissue absorption | Antigen absorption | Unabsorbed | Tissue absorption | |||||||
| Cortex | SVZ | Cortex | SVZ | Cortex | SVZ | Cortex | SVZ | Cortex | SVZ | ||
| Secondary antibodies | Abcam 150072 (Unabsorbed) | ++++ | +++++ | ± | ++++ | ++ | – | +++ | ++++ | + | ++++ |
| Abcam 150072 (Absorbed) | +++ | +++++ | ± | ++++ | + | – | ++ | +++ | ± | +++ | |
| Thermo A11036 (Unabsorbed) | +++ | ++++ | – | ++++ | ++ | – | ++ | ++++ | ± | ++++ | |
| Thermo A11036 (Absorbed) | +++ | ++++ | – | +++ | + | – | ++ | +++ | ± | ++++ | |
| Abcam 150112 (Unabsorbed) | |||||||||||
| Abcam 150112 (Absorbed) | |||||||||||
| Thermo A11031 (Unabsorbed) | |||||||||||
| Thermo A11031 (Absorbed) | |||||||||||
| Primary antibodies/disposition/brain region | |||||||||||
| Abcam 18723-2 (Lot.GR3224908-1) | CST-4604 | SC-271390* | |||||||||
| Antigen absorption | Unabsorbed | Tissue absorption | Unabsorbed | Tissue absorption | |||||||
| Cortex | SVZ | Cortex | SVZ | Cortex | SVZ | Cortex | SVZ | Cortex | SVZ | ||
| Secondary antibodies | Abcam 150072 (Unabsorbed) | ++ | – | + | ++++ | ± | ++++ | ||||
| Abcam 150072 (Absorbed) | + | – | ± | ++++ | – | ++++ | |||||
| Thermo A11036 (Unabsorbed) | + | – | ± | ++++ | – | ++++ | |||||
| Thermo A11036 (Absorbed) | + | – | – | ++++ | – | ++++ | |||||
| Abcam 150112 (Unabsorbed) | ± | ++++ | – | ++++ | |||||||
| Abcam 150112 (Absorbed) | – | ++++ | – | ++++ | |||||||
| Thermo A11031 (Unabsorbed) | ± | ++++ | – | ++++ | |||||||
| Thermo A11031 (Absorbed) | – | ++++ | – | ++++ | |||||||
*There were no significant differences between SC-271390 and SC-8066. “+, ± and –”: Fluorescence intensity from observation and spectrometric determination. “±” Indicates that the outlines of the perikarya were weakly immunolabeled or that the immunofluorescence was only slightly above background in subsequent quantitative spectroscopy examinations. DCX: Doublecortin; SVZ: subventricular zone.