Figure 2.
TA-8 inhibited lipopolysaccharide (LPS)-induced HMGB1-NF-κB translocation in RAW264.7 cells. (A) TA-8 can inhibit NF-κB transcriptional activity; (B–E) TA-8 can inhibit nuclear translocation and phosphorylation of NF-κB P65, RAW264.7 cells were pretreatment with TA-8 (10−5, 10−6, 10−7, and 10−8 mol/L) for 40 min, and then were stimulated with LPS (1 μg/ml) for 45 min. The nuclear transfer of p65 was detected by using inverted fluorescence microscopy. The level of P65 (nucleus), P65, p-P65, and GAPDH were examined using a specific antibody. (F–I) TA-8 can inhibit LPS-induced IκBα phosphorylation and up-regulation of TLR4-IKBKB expression. (J–L) TA-8 can significantly inhibit the decrease of HMGB1 content induced by LPS and inhibit the secretion of HMGB1 into cytoplasm. # P < 0.05 versus the control group; * P < 0.05, versus LPS-stimulated cells.