Table 1.
List of Antigens, genes, and released proteins used in these studies.
| Flow antibodies | qPCR primers | Multiplex | |||||
|---|---|---|---|---|---|---|---|
| Molecule | Alternate name | Antibody | Clone | Company | Sense | Antisense | |
| CD16 | FcγRIII | CD16/32-PECy7 | 2.4G2 | BD Pharmingen | ACTGTCCAAGACCCAGCAACTAC | GCACATCACTAGGGAGAAAGCA | |
| CD32 | FcγRIIb | GCCAAAACTGAGGCTGAGAATAC | CAGGGCTTCGGGATGCT | ||||
| CD64 | FcγRI | CD64-PE | 290322 | R&D Systems | AGATGCTGGATTCTACTGGTGTGA | TGTGAAACCAGACAGGAGCTGAT | |
| CD16.2 | FcγRIV | CD16.2-BV421 | 9E9 | Biolegend | ACAAATCTTCAGCATCCTTTCGTAT | CGGTGGAAACATGGATGGA | |
| CD119 | IFN-γ Receptor | CD119-PE | 2.00E+02 | eBiosciences | — | — | |
| CD206 | Mannose Receptor | CD206-FITC | MR5D3 | AbD Serotec | — | — | |
| CD124 | IL-4R (α subunit) | CD124-PECy7 | I015F8 | Biolegend | — | — | |
| MHCII | MHC Class II | MHCII (I-A/I-E)-APC | M5/114.15.2 | eBiosciences | — | — | |
| Ly6C | Ly6C-PECy7 | HK1.4 | Biolegend | — | — | ||
| Ly6G | Ly6G-APC | 1A8 | Biolegend | — | — | ||
| SiglecF | CD170 | SiglecF-PE | E50-2440 | BD Pharmingen | — | — | |
| F4/80 | Adgre1 | F4/80-FITC | A3-1 | AbD Serotec | — | — | |
| CD11b | β2 Integrin (α chain) | CD11b-eFluor 450 | M1/70 | eBiosciences | — | — | |
| TLR2 | CD282 | TLR2-FITC | 6C2 | eBiosciences | — | — | |
| TLR4 | CD284 | TLR4-APC | SA15-21 | Biolegend | — | — | |
| CCL2 | MCP-1 | — | — | — | — | — | + |
| CCL5 | RANTES | — | — | — | — | — | + |
| CCL11 | Eotaxin | — | — | + | |||
| CXCL1 | KC | — | — | — | — | — | + |
| Arginase I | Arg-1 | — | — | — | GGAAAGCCAATGAAGAGCTG | GCTTCCAACTGCCAGACTGT | |
| Inducible nitric oxide synthase | iNOS | — | — | — | TCTATCAGGAAGAAATGCAGG | CACCAGCTTCTTCAATGTGG | |
| Interluekin-1β | IL-1β | — | — | — | AATGAAAGACGGCACACCC | GCTTGTGCTCTGCTTGTGA | + |
| Interleukin-6 | IL6 | — | — | — | AAGACAAAGCCAGAGTCC | CCTTCTGTGACTCCAGCTT | + |
| Interleukin-10 | IL10 | — | — | — | TGTGAAAATAAGAGCAAGGCAGTG | GCCTTGTAGACACCTTGGT | + |
| Interleukin-12 p40 | IL12 (p40) | — | — | — | AGCACTCCCCATTCCTACTT | CACGCAGACATTCCCGCC | |
| Interleukin-12 p70 | IL-12 (p70) | — | — | — | — | — | + |
| Tumor Necrosis Factor α | TNF-α | — | — | — | — | — | + |
| β-actin | — | — | — | TTCCAGCCTTCCTTCTTGG | AGTAATCTCCTTCTGCATCC | ||