Table 2.

Comparison of surface expression in BMDM and pMACs in response to polarization.

Protein	Cell type main effect	Polarization main effect	Interaction effect
CD11b	0.213	0.343	0.698
F4/80	0.910	0.438	0.189
Ly6G	0.393	0.229	0.914
Ly6C	0.009	0.005	0.008
MHCII	0.813	<0.001	0.867
TLR2	0.002	<0.001	0.040
TLR4	<0.001	<0.001	<0.001
CD64	0.077	0.001	0.112
CD16/32	0.802	<0.001	0.819
CD16.2	<0.001	<0.001	<0.001
CD119	0.836	<0.001	0.080
CD124	0.001	0.008	0.005

BMDM and pMACs were polarized as described in Methods. Cell surface expression was quantified as Mean Fluorescent Intensity by flow cytometry as in Figure 3 and the statistical significance based on cell type (irrespective of polarization), polarization (irrespective of cell type), or cell type and polarization (interaction effect) determined. Specifically, the deviation from the mean, induced by IFN-γ, IL4/IL13, or left untreated (NT) was calculated for each animal (and graphed in Figure 7). Statistical significance was determined by 2-way ANOVA, thus removing the animal-to-animal variability and considering only the within-animal responses. p-values are presented. Statistically significant differences are indicated in bold text. n = BMDM and pMACs from 7 animals.