Table 3.

Comparison of mRNA expression from BMDM and pMACs in response to polarization.

Gene	Cell type main effect	Polarization main effect	Interaction effect
IL-12 p40	0.382	0.035	0.358
IL-10	0.099	0.019	0.001
Arg-1	0.757	<0.001	<0.001
iNOS	0.868	0.088	0.752
IL-6	0.185	0.395	0.644
IL-1β	0.017	0.088	0.178
TGF-β	0.444	0.405	0.990
CD206	0.283	<0.001	0.233

BMDM and pMACs were polarized as described in Methods and expression of the indicated genes quantified by qPCR and reported as fold change (Figure 7). After logarithmic transformation, those data were fit with a linear model and statistical significance determined by ANOVA (α = 0.05) based on cell type alone (irrespective of polarization), polarization only (irrespective of cell type), or a differential response dependent on both cell type and polarization conditions (interaction effect). p-values are presented. Statistically significant differences are indicated in bold text. n = BMDM and pMACs from 4–6 animals.