Table 4.

Comparison of cytokine/chemokine release in BMDM and pMACs in response to polarization.

Protein	Absolute concentration			Normalized protein release
	Cell type main effect	Polarization main effect	Interaction effect	Cell type main effect	Polarization main effect	Interaction effect
IL-10	<0.001	0.001	0.001	<0.001	<0.001	<0.001
IL-12 p40	0.02	0.001	0.664	0.574	<0.001	0.545
IL-6 (NSD from 1)	0.362	0.247	0.692	0.281	0.092	0.959
CXCL1/KC	0.386	<0.001	0.026	<0.001	<0.002	<0.003
CCL5/RANTES	0.019	0.082	0.635	0.077	0.016	0.528
CCL2/MCP-1	0.002	<0.001	<0.001	0.105	<0.001	<0.001

BMDM and pMACs were polarized as described in Methods, the media was collected and secretion of the indicated genes quantified by Multiplex®. The released protein (Figure 9) was either fit with a linear model (Left columns) or normalized to their respective controls, logarithmically-transformed, and fit with a linear model (Right columns) and analyzed by ANOVA (α = 0.05). Significance was based on cell type (irrespective of polarization), polarization (irrespective of cell type), or a differential response dependent on both cell type and polarization (interaction effect). Statistically significant differences based on cell type, polarization, or both are indicated in bold text. n = BMDM and pMACs from 4–7 animals. The interaction significance indicates that BMDM and pMACs respond differently to polarization. Of the genes tested, the interaction effect for IL-10, CXCL1, and CCL2 is significant (bold text).