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. 2020 Feb 21;11:119. doi: 10.3389/fphys.2020.00119

FIGURE 3.

FIGURE 3

(A) Alveolar wall thickness expressed a score out of 4, (B) Immune cell infiltration expressed as the number of positive cells per field of view, (C) number of proliferating cells expressed as the percentage of positive cells. Scale bars show 50 μm. *P < 0.05, **P < 0.01, ****P < 0.0001 compared to controls. (D–F) Representative lung sections stained with Hematoxylin and Eosin to assess alveolar wall thickness in (D) control, (E) VILI, and (F) VILICELLS lambs. (G–I) Representative lung sections demonstrating immune cell presence using CD45 immunohistochemistry. (J–L) Representative lung sections demonstrating cellular proliferation using Ki67 immunohistochemistry. Scale bar shows 30 μm.