FIGURE 4.

(A) %Elastin content in tissue. (B) Density of secondary septal crests expressed as number per tissue area. (C) %Collagen content in tissue. *P < 0.05, **P < 0.01 compared to controls. (D–L) Representative lung sections stained with HARTs to view elastin (black staining) in (D,G) control (E,H) VILI, and (F,I) VILI_CELLS. Arrows indicate elastin deposition at the points of septa in control lambs, which is irregular in the VILI and VILI_CELLS lambs. (J–L) Representative lung sections stained with picrosirius red to view collagen (dark red staining).