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. 2020 Feb 24;14:731–744. doi: 10.2147/DDDT.S231916

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© 2020 Hang et al.

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Ox-LDLs upregulated the expression of ASK1, ERS -and inflammasome-related proteins in a dose-dependent manner. Endothelial cells were treated with different concentrations of ox-LDL. (A) ASK1 and p-ASK1 expression were measured by Western blotting (B) The expression of ERS-related proteins was examined by Western blotting. (C) Western blotting was used to investigate the expression of inflammasome-associated proteins. (D) The concentrations of IL-1β and IL-18 were examined by ELISA. (E) LDH levels were evaluated using a LDH-Cytotoxicity Assay Kit. *P < 0.05, **P < 0.01, ***P < 0.001 vs control group.