Table 3. Detection of transgene fragments in stool samples by TaqMan qPCR and SYBR Green qPCR.
| Method | Group | Copy/μL of Transgene | ||||
|---|---|---|---|---|---|---|
| pre | 6 h | 12 h | 24 h | 48 h | ||
| Taqman qPCR | IV | 0 | 30.0 ± 11.4** | 85.4 ± 39** | 967.5 ± 388.3** | 10.0 ± 1.4** |
| IM | 0 | 15.1 ± 4.9** | 85.8 ± 30.8** | 913.9 ± 263** | 43.2 ± 12.2** | |
| IP | 0 | 1,809.6**±1,587.9 | 180.8 ± 148.1** | 0 | 0 | |
| SYBR qPCR | IV | 426.9 ± 159.8 | 65.4 ± 16.6 | 111.2 ± 18.3 | 239.9 ± 62.5 | 34 ± 5.4 |
| IM | 762.4 ± 239.3 | 44 ± 5.7 | 117.7 ± 23.6 | 263.8 ± 56.1 | 46.1 ± 7.1 | |
| IP | 885.1 ± 403.6 | 1,111.6 ±965.2 | 103.8 ± 35 | 110.1 ± 10.2 | 51.6 ± 8.4 | |
Notes.
Transgene fragments were detected in all samples in TaqMan qPCR.
- IV
- intravenous injection
- IM
- intramuscular injection
- IP
- intraperitoneal injection of hEPO coding naked plasmid
**
p < 0.01 vs Pre in each group.
*
p < 0.05 vs Pre in each group.