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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Biosens Bioelectron. 2020 Jan 22;153:112042. doi: 10.1016/j.bios.2020.112042

Figure 1.

Figure 1.

(A) Dual-layer paper chip assay procedure with 1) a capture layer (glass fiber), pre-loaded with red fluorescent, anti-ROR1 conjugated microparticles, 2) a flow layer (wax-printed chromatography paper), 3) a buffy coat sample with dosed cancer cells, and 4) a blunt-end needle tip syringe for uniform droplet application. (B) A smartphone captures a video for monitoring the flow velocity from the flow layer. (C) A smartphone-based fluorescence microscope images the capture layer for quantifying ROR1+ cancer cell. (D) Schematic of flow-based detection with no target (more particles at the flow front, increasing interfacial tension γLG) and with target (more particles not at the flow front due to immunoagglutination, increasing viscosity μ).