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. 2020 Feb 21;8:125. doi: 10.3389/fbioe.2020.00125

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Copyright © 2020 Lü, Ge, Cao, Guo, Liu, Gao, Xu and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time-course of batch fermentation by B. licheniformis MW3 (ΔbudCΔgdh). , Glucose; , OD_620nm; , 2,3-butanediol; , acetoin. The experiment was conducted in a 1-L bioreactor (Infors AG, Bottmingen, Switzerland) with 0.8 L initial medium with an initial glucose concentration of 70 g/L approximately. Cultivation was carried out at 50°C and an initial pH of 7.0. The pH was maintained at 7.0 by automatic addition of 6 M acetic acid and 6 M NaOH. The agitation speed was 500 r/min and the aeration rate was 1 vvm. Data were the means ± SDs from three parallel experiments.

Inline graphic — Time-course of batch fermentation by B. licheniformis MW3 (ΔbudCΔgdh). , Glucose; , OD_620nm; , 2,3-butanediol; , acetoin. The experiment was conducted in a 1-L bioreactor (Infors AG, Bottmingen, Switzerland) with 0.8 L initial medium with an initial glucose concentration of 70 g/L approximately. Cultivation was carried out at 50°C and an initial pH of 7.0. The pH was maintained at 7.0 by automatic addition of 6 M acetic acid and 6 M NaOH. The agitation speed was 500 r/min and the aeration rate was 1 vvm. Data were the means ± SDs from three parallel experiments.