FIGURE 4.

Protein lysine acetylation in MG1655, KBM1081, KBM1082, and KBM1084. Shown are Western Blots of gels probed with Acetylated Lysine Multi MAB with extracts from exponentially growing aerobic cultures (A) and anaerobic cultures (B). (C) Shows the Coomassie stained loading control for the anaerobic extracts used in (B). For the Western Blots shown in (A,B) 15 μl of the respective extracts were loaded per lane. For the loading control in (C) only 5 μl of the extracts were loaded. As markers MagicMark XP Western Protein Standard (Thermo Fisher Scientific) was used (A) and Precision Plus Unstained Protein (BioRad) for (B,C). Culture growth and extract preparation were performed as described in Section “Materials and Methods.”