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. 2020 Feb 21;11:233. doi: 10.3389/fmicb.2020.00233

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Copyright © 2020 Schütze, Benndorf, Püttker, Kohrs and Bettenbrock.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Anaerobic growth of the ackA mutant KBM1081 in defined medium supplemented with uracil and methionine. Shown are time course data from representative growth assays for growth of the ackA mutant KBM1081 in defined medium with 4 g/l glucose as carbon source without any further growth supplements (black) and with addition of 30 μg/ml uracil (red) and with addition of 30 μg/ml uracil plus 40 mg/l L-methionine (green). Growth of the ackA-pta mutant KBM1084 with supplementation of uracil and methionine is shown for comparison (gray).