Figure 2.

Decision chart and overview of the main considerations for project design in high throughput sequencing. The flow chart shows the most common groups of sequencing methodologies. Sections 1–3 summarize key components of project design, starting by choosing the sequencing methods, followed by bait design and finishing with the optimization of laboratory practices. Section 3 shows recommended (full circle), recommended in some cases (half circles) and not recommended (empty circles) practices based on input DNA quality and quantity. “Low input” refers to low input DNA extraction kits and “touch down” refers to temperature ramps at the hybridization and capture steps.