Skip to main content
. 2020 Feb 21;8:100. doi: 10.3389/fbioe.2020.00100

Figure 3.

Figure 3

Cell viability studies in RAW 264.7 cell line. (A) Cell viability decrease induced by different concentration of Chit 80% NPs, Chit 93% NPs, Chit 80%, and Chit 93% polymers evaluated by MTT assay after 24 h incubation. Dotted line represents the 70% of cell viability. (B) Nonlinear regression analysis of the cell viability data, allowing the extrapolation of IC50 values (4,949 μg/mL for Chit 80% NPs and 4,858 μg/mL for Chit 93% NPs). No statistical difference was found between Chit 80% NPs IC50 and Chit 93% NPs IC50 calculated using extra sum-of-squares F test. (C) Evaluation of possible NP and polymer interference with the wavelengths used to read MTT assay (540/630 nm) (Mean ± SEM, n = 3). (D) Evaluation of the cell viability resultant from the incubation of RAW 264.7 with the NPs solvent and polymers solvent (% of control). Results are expressed as mean ± SEM, obtained from a minimum of three independent experiments, each performed in triplicate (n ≥ 3).