Skip to main content
. 2020 Feb 21;8:100. doi: 10.3389/fbioe.2020.00100

Figure 7.

Figure 7

Hemolysis assay. (A) Hemolytic activity of Chit polymers and NPs in human blood after 3 h incubation at 37°C. PBS and Triton-X-100 were respectively used as negative (C–) and positive control (C+). Results are expressed as mean ± SEM, obtained from at least three independent experiments, using blood from different donors, each performed in duplicate (n ≥ 3). (B) Representation of 100% hemolysis generated by the positive control (tube 1 and 2) and the absence of hemolysis induced by the negative control (tube 3 and 4). (C) Evaluation of the Chit NP and Chit polymers interferences with the absorbance readout, without blood. Results are expressed as mean ± SEM, obtained from at least three independent experiments, using blood from different donors, each performed in duplicate (n ≥ 3). (D) Evaluation of the hemolysis resultant from the incubation of NPs solvent and polymers solvent in human blood after 3 h incubation at 37°C.