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. 2020 Feb 21;8:100. doi: 10.3389/fbioe.2020.00100

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Copyright © 2020 Jesus, Marques, Duarte, Soares, Costa, Colaço, Schmutz, Som, Borchard, Wick and Borges.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Coagulation assay. (A) Effect of Chit NPs and polymers at 0.1 and 1 mg/mL on plasma coagulation time after incubation for 30 min. The two coagulation pathways, APTT and PT, were separately tested. APTT reference range of values is 20–40 s and for PT is 11–14 s. Results are expressed as mean ± SEM, obtained from three independent experiments, using blood from different donors, each performed in duplicate (n ≥ 3). (B) Controls of interferences of NPs and polymers solvents with the coagulation times assay. Results are expressed as mean ± SEM, obtained from three independent experiments, using blood from different donors, each performed in duplicate (n ≥ 3).