Skip to main content
. 2020 Feb 21;8:100. doi: 10.3389/fbioe.2020.00100

Figure 9.

Figure 9

Effect of Chit 80% NPs and Chit 93% NPs in platelet aggregation. Platelet aggregation was detected by incubating PRP with 2 mg/mL of NPs for 15 min. PBS, collagen (200 and 600 μg/mL) and calcium chloride (CaCl2, 0.25 M) were used as negative and positive control, respectively. (A) Representative images of platelet aggregation assay stained with Giemsa dye. Untreated platelet free plasma (PFP) is represented in image 1 and untreated platelet rich plasma (PRP) is represented in image 2. For the experiment two different positive controls were used (CaCl2–3 and collagen−4). Chit 80% NPs and Chit 93% NPs were tested both with PFP (image 5 and 7) and PRP (image 6 and 8). (B) Quantification of the platelet aggregation effect. Platelet count is presented as the final average of a minimum of three donors ± SD. The percentage of aggregation was calculated using as reference the platelet count of the negative control, and is presented as the average of all assays ± SD (n ≥ 3).