Table 1.

Physicochemical characterization of Chit polymers and NPs. (A) Polymer molecular weight (MW), deacetylation degree (DDA), and size in acetate buffer and after resuspension in DMEM and RPMI at 37°C (Mean ± SEM). (B) Chit 80% and Chit 93% NPs size, polydispersity index and zeta potential (ζ), in water and after resuspension DMEM and RPMI at 37°C (Mean ± SEM). (C) Endotoxin contamination evaluated with the Pyrochrome^® kit for Chit 80% NPs, Chit 93% NPs, Chit 80%, and Chit 93% and TPP solution. Endotoxin contamination of pyrogen-free water was also evaluated for comparison. Mean ± SEM; n = 3 (three different batches).

A
	MW (kDa) (n = 1–3)		DDA (%) (n = 1)		Size (μm) (n ≥ 3)
					Acetate buffer	DMEM		RPMI
	Non-purified	Purified	Non-purified	Purified		1 h	24 h	1 h	24 h
Chit 80%	168	49	78	80	612 ± 40	529 ± 39	541 ± 49	628 ± 94	479 ± 58
Chit 93%	127	122	94	93	608 ± 23	590± 37	518 ± 57	555 ± 50	525 ± 53