B
		Water	DMEM		RPMI
			1 h	24 h	1 h	24 h
Chit 80% NPs	Size (nm)	127 ± 5	109 ± 29	133 ± 22	116 ± 29	368 ± 141
(n ≥ 3)	PDI	0.28 ± 0.01	0.72 ± 0.03	0.62 ± 0.11	0.47 ± 0.08	0.52 ± 0.03
	ζ (mV)	+29.0 ± 1.0	−4.9 ± 0.2		−2.1 ± 0.4
Chit 93% NPs	Size (nm)	292 ± 52	106 ± 20	147 ± 74	321 ± 48	327 ± 131
(n ≥ 3)	PDI	0.18 ± 0.03	0.49 ± 0.14	0.48 ± 0.21	0.91 ± 0.13	0.76 ± 0.16
	ζ (mV)	+20.0 ± 6.0	−3.9 ± 0.6		−4.4 ± 0.5