Figure 2.

Approach to IVD fabrication and testing. A) Schematic process flow: Cy5-streptavidin printed onto POEGMA-coated glass slides using u-AJP. Deposition dried overnight at 30°C, then incubated in a dilution series of AF488-biotin and rinsed in 0.1% Tween-20/PBS then dried by centrifugation for 10 seconds. Binding event of AF488-biotin to Cy5-streptavidin is characterized by fluorescence. A representative florescent image and cartoon of the fluorescent intensity from a printed feature that is blank (B) and one with significant binding events (C).