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. Author manuscript; available in PMC: 2020 Feb 28.
Published in final edited form as: Biofabrication. 2020 Feb 4;12(2):025004. doi: 10.1088/1758-5090/ab5cf5

Figure 3:

Figure 3:

Confirmation of preserved biofunctionality following u-AJP printing and comparison of large and small spot sizes using streptavidin-biotin assay. A) i. Printed spots of Cy5-streptavidin measured at 647 nm, then similar spots observed at 488 nm after: ii. treatment with FITC-conjugated biotin showing active binding, and iii. rinsing with PBS control without any biotin incubation. B) Dose-response curve from AJP-printed assay of streptavidin-biotin. Data represent average ± SD of 3 separately run assays. C) 3D (left) and 2D (right) images of FITC-biotin-treated single spots from the AJP with a 22 μm (top) and 130 μm diameter (bottom), imaged at 488 nm.