Figure 2.

Knockdown of miR-486-5p enhanced 5-Fu-induced apoptotic rate in PANC-1 cells. PANC-1 cells were transfected with 10 nM miR-486-5p antagonist and then exposed to 5-Fu for 48 hrs. Meanwhile, PANC-1 cells were transfected with 10 nM miR-486-5p antagonist or exposed to 5-Fu for 48 hrs, respectively. (A, B) Apoptotic cells were detected with Annexin V and PI double staining. (C) Expression levels of Bax, cleaved caspase 3 and Bcl-2 in PANC-1 cells were detected with Western blotting. (D–F) The relative expression of Bax, cleaved caspase 3 and Bcl-2 in PANC-1 cells were quantified via normalization to β-actin. *P<0.05, **P<0.01 vs control group; ^##P<0.01 vs 5-Fu group.