Figure 3.

Downregulation of miR-486-5p enhanced the inhibitory effects of 5-Fu on the migration and invasion of PANC-1 cells. PANC-1 cells were transfected with 10 nM miR-486-5p antagonist and then exposed to 5-Fu. Meanwhile, PANC-1 cells were transfected with 10 nM miR-486-5p antagonist or exposed to 5-Fu, respectively. (A, B) Wound healing assay was used to detect the cell migration. Representative images captured at 0 and 48 h are shown (magnification x 400). (C, D) Transwell invasion assay was used to measure the invasive ability of PANC-1 cells. *P<0.05, **P<0.01 vs control group; ^##P<0.01 vs 5-Fu group.