Figure 6.
Downregulation of miR-486-5p enhanced the anti-tumor effect of 5-Fu in PANC-1 xenograft model in vivo. (A) 1 × 107 PANC-1 cells were injected subcutaneously into the left flank of nude mice. 50 nM miR-486-5p antagonist was directly injected into the tumors twice a week. Meanwhile, the mice were intraperitoneal administration of 5-Fu weekly. The body weight of mice was monitored. (B) The xenograft tumor volumes were monitored weekly. (C) Xenografts tumors were photographed after 4 weeks. (D) The weights of the tumors were calculated. (E) RT-qPCR was used to detect the level of miR-486-5p in tumor tissues. (F) Expression level of PTEN in tumor tissues was detected with Western blotting. (G) The relative expression of PTEN in tumor tissues was quantified via normalization to β-actin. *P<0.05, **P<0.01 vs control group; ##P<0.01 vs 5-Fu group.