Figure 3. Immunophenotype of normal dog PBMC with caCD94, CD3, CD5 and NKp46.

Live mononuclear cells (FSC^loSSC^lo) were placed into and NKp46 by CD5 gate (center scatter-gram). CD5^hiNKp46^– are almost entirely CD3⁺, (top right scatter-gram). CD5⁺intermediate NKp46⁺ are mostly almost entirely CD3⁺, but with a high CD94⁺ population (ostensibly NKT cells) and a small portion CD94^– (center right scatter-gram). In the CD5 dim NKp46^hi population almost all cells are CD3^–CD94^hi putative NK cells, (lower right scatter-gram).