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. 2020 Feb 28;21:62. doi: 10.1186/s12931-020-1322-5

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Identification of HDAC8 and Gal-3 interaction. HDAC8 and Gal-3 (a) in protein complex from cell lysate of macrophages was immunoprecipitated (IP) with anti-HDAC8 and anti-IgG, identified by immunoblotting (IB). Input (lysates) was loaded with same amount of protein in sample. An isotype IgG was a negative control. The expression levels of Gal-3 (b) and Arg1 (c) in macrophages were determined by shRNA and PCI. The results were expressed as Mean ± SD (n = 3). **: P < 0.01 vs RNase free H₂O, IL-4/shRNA and IL-4/PCI. #: P < 0.05 vs the control (n = 3)