Fig. 1.

Secretion of AmyL, AmyE and BPN’ upon 16, 20 or 24 h of growth. Cells were separated from the growth medium by centrifugation after 16, 20 or 24 h of growth in MBU medium at 37 °C. Subsequently, proteins in the growth medium fractions were precipitated with TCA, separated by LDS-PAGE, and visualized with SimplyBlue SafeStain (upper panel). Prior to TCA precipitation and gel loading, the samples were corrected for the OD₆₀₀ of the respective cultures as listed in the bottom panel. To assess the extent of cell lysis during culturing, the extracellular levels of the cytoplasmic marker protein TrxA were assessed by Western blotting with specific antibodies (middle panel). Molecular weights of marker proteins are indicated (in kDa) on the left side of the gel segment