Fig. 6.

Analysis of HtrA and HtrB levels in strains lacking individual non-essential secretion machinery components or cell envelope-associated proteases upon production of AmyE or AmyL. The levels of full-size HtrA (a) or HtrB (b) in wild-type or mutant cells producing AmyE or AmyL was assessed by Western blotting with specific antibodies as described for Fig. 5. The relative levels of HtrA or HtrB compared to the respective levels in the wild-type strain were assessed by ImageJ analysis. Black bars represent the HtrA or HtrB levels in non-producing strains, grey bars relate to the HtrA or HtrB levels in AmyE-producing strains, and white bars to HtrA or HtrB levels in AmyL-producing strains. The error bars represent the standard error of the mean for three independent experiments