Table 1.
Sequence conservation and microtubule interaction properties among TgDCX and its orthologues
| orthologue | DCX domain | P25α-DCX domain | P25α to DCX1 | N-term. extension2 | T. gondii location3 | Xenopus4 |
|---|---|---|---|---|---|---|
| (size, aa) | (identical/similar, %) | (identical/similar, %) | (aa) | (aa) | ||
| TgDCX (256) | (100/100) | (100/100) | 44 | 62 | A,D,T | B,B,B |
| CvDCX1 (213) | 46/65 | 50/60 | 44 | 31 | A,D,T | B,B |
| CvDCX2 (174) | 33/54 | 36/52 | 38 | 4 | a,D,C | |
| CvDCX3 (175) | 32/53 | 35/52 | 38 | 3 | a,D,C | |
| VbDCX1 (190) | 48/71 | 47/63 | 39 | 14 | a,D,c5 | |
| VbDCX2 (168) | 39/56 | 39/53 | 45 | 8 | a,D,C | |
| VbDCX3 (184) | 39/56 | 41/56 | 45 | 0 | a,D,C | |
| PfDCX (238) | 45/71 | 36/56 | 35 | 83 | a,D,C | B,B |
| TaDCX (178) | 34/53 | 36/51 | 38 | 7 | C | b |
1: Length of linker between the P25α domain and the DCX domain
2: Length of sequence preceding the P25α domain
3: Localization in Toxoplasma of the protein when expressed under control of the α-tubulin promoter is specified as: A, adult (mother) conoid strongly; a, adult conoid weakly; D, daughter conoid; T, daughter cortical MT; C or c, bright or dim diffuse cytoplasmic
4: Localization in Xenopus S3 cells is specified as: B or b, binds to microtubules prominently or with high cytoplasmic background; B,B, binds to and bundles microtubules; B,B,B, binds, bundles, and bends microtubules
5: Also prominent mitochondrial localization