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. 2020 Feb 28;39:44. doi: 10.1186/s13046-020-1541-0

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© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — FKBP9 depletion inhibits the malignant phenotypes of GBM cells in vitro. a Efficiencies for stable knockdown in LN-229, SF-539 and T98G cells of FKBP9 (indicated as LN-229-shControl, LN229-shFKBP9; SF-539-shControl, SF-539-shFKBP9; T98G-shControl, T98G-shFKBP9) were tested by IB assays. IB analysis for the rescue efficiency of adenoviruses overexpressing FKBP9 in T98G-shFKBP9 cells using GAPDH as a loading control (MOI = 1 or 10). b-d LN-229-shFKBP9, SF-539-shFKBP9 and T98G-shFKBP9 cells were introduced with adenoviruses carrying the vector control (Ad-vector) and adenoviruses overexpressing FKBP9 (Ad-FKBP9). Analysis of cell viability, colony formation and invasion of these cells was performed. Protein levels of Bcl-2, XIAP and Mcl-1, N-Cadherin and Vimentin were detected by IB assays. e Analysis of the ability of LN-229-shFKBP9 and SF-539-shFKBP9 cells with or without Ad-FKBP9 to form spheres in 3D cultures. Scale bar = 200 μm (× 10). Number and size of spheres were counted and measured. Stem markers including Sox2 and Oct4 of LN-229 cells spheres were detected by IB assays. All experiments were performed three times with comparable results. Data are represented as mean ± S.D. (*p < 0.05, **p < 0.01, ***p < 0.001)