Figure 1.

Effect of ethylparaben on cell morphology and viability of BeWo cells. (A) BeWo cell viability was measured using the MTS assay. Cells were treated with 0.1–10 mM of ethylparaben for 24 or 48 h. Data are presented as the percentage of the values obtained for the untreated cells used as the control. (B) Cell morphology was observed using phase-contrast microscopy at the indicated time points, for various concentrations of ethylparaben. (C) Cell viability was analyzed using the live/dead assay. Cells were stained with two dyes (Calcein-AM, 0.3 μM; EthD-1, 3 μM) for 30 min at room temperature (approximately 25°C) and stained cells were detected by fluorescence microscopy. Images represent three independent experiments. Scale bars represent 200 μm.