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. 2020 Jan 9;24(1):34–43. doi: 10.1080/19768354.2020.1711804

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group Korean Society for Integrative Biology

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Ethylparaben treatment reduced the expression of Cyclin D1 protein in BeWo cells. The effect of ethylparaben treatment on the protein expression level of Cyclin D1 was analyzed using flow cytometry. BeWo cells were treated with 0.1–1 mM of ethylparaben for 24 or 48 h. Cells were fixed with 1% PFA and were stained with the antibody against Cyclin D1 (upper panel). Data are expressed as the percentage of Cyclin D1 positive cells (lower panel). Data represent the mean ± S.E.M. of three independent experiments (n = 6) measured in triplicate (*, p < .05; **, p < .01; ***, p < .001 compared with cells treated with 0.6% EtOH as a negative control).