Figure 6.

Cleaved Caspase-3 level was increased by ethylparaben treatment in BeWo cells. Functional Caspase-3 level after treatment with ethylparaben was analyzed by the detection of cleaved forms of Caspase-3 using flow cytometry. After treatment with 0.1–1 mM of ethylparaben for 24 or 48 h, BeWo cells were fixed with 1% PFA and stained with the antibody against cleaved-Caspase-3 (c-Caspase-3; upper panel). Data are expressed as the percentage of c-Caspase-3 positive cells (lower panel). Data represent the mean ± S.E.M. of three independent experiments (n = 6) measured in triplicate (***, p < .001 compared with cells treated with 0.6% EtOH as a negative control).