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. Author manuscript; available in PMC: 2020 Jun 11.
Published in final edited form as: Nature. 2019 Dec 11;576(7787):477–481. doi: 10.1038/s41586-019-1831-x

Extended Data Figure 3 |. Plasma membrane v-ATPase regulates cholesterol distribution and macropinocytosis.

Extended Data Figure 3 |

a, Fluorescence micrographs of HeLa Ctl and HeLa HV12 or T7-KRasV12 (KV12) cells immunostained with anti-V1A antibody. b, Validation of V1A antibody for immunofluorescence: fluorescence micrographs of V1A immunostaining of HeLa HV12 cells transfected with the indicated siRNAs. c, Fluorescence micrographs of V1A immunostaining and filipin labeling of HeLa KV12 cells transfected with the indicated siRNAs. d, Quantification of TMR-dextran uptake in mutant Ras cell lines transfected with siV0a3 in the presence or absence of exogenous cholesterol. Images (a-c) are representative of three biological replicates. Scale bars, 10μm. For b,c, dashed lines delineate the cell and/or nucleus. For a,c, the mean percentages ±s.e.m shown represent the fraction of cells that display V1A PM localization. ≥500 (a,c,d) cells were quantified in each biological replicate (n=3) with error bars indicating mean ±s.e.m. where shown; p value: unpaired, two-tailed Student t-test.