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. 2020 Jan 10;53(2):e12758. doi: 10.1111/cpr.12758

Figure 4.

Figure 4

Isolation and genotypic identification of mouse embryonic EMSCs. E13.5 heterozygous mice were selected and each embryo was separated (A, B). The embryonic maxillofacial processes were dissected and minced into fine pieces for the culture of EMSCs (C‐F). EMSCs exhibited a fibroblast‐like morphology (G, H). Genotypic identification was shown for each embryo used to isolate EMSCs (I). Scale bar represents 50 μm. Abbreviations: H, heterozygous; K, knockout; W, wild type