Figure 7.
Investigations revealing the potential mechanism of p75NTR in mineralization. Immunohistochemistry showed similar expression patterns for p75NTR and Mage‐D1 (A). They were strongly expressed in the same areas at the cap stage (E13.5 d), but their expression became weak at the bell stage (E15.5 d) and at the beginning of dental hard tissue formation (E18.5 d). Western blot showed that Runx2 was significantly decreased when Mage‐D1 was down‐regulated by siRNA transfection (B). The homeobox genes Dlx1 and Msx1 were both weakly expressed in p75NTRExIII−/− EMSCs but strongly expressed in p75NTR+/+ EMSCs (C). Abbreviations: K, knockout; W, wild type