Table 1.

Specific primers used for RT‐PCR

Gene	Primer sequences	GenBank® Accession no.
OPN	Forward: 5′‐TTCTCCTGGCTGAATTCTGAGG‐3′ Reverse: 5′‐GCTATAGGATCTGGGTGCAGG‐3′	NM_001204203
OCN	Forward: 5′‐CTTGGTGCACACCTAGCAGA‐3′ Reverse: 5′‐GCCGGAGTCTGTTCACTACC‐3′	X04142
BSP	Forward: 5′‐GGCGACACTTACCGAGCTTA‐3′ Reverse: 5′‐GGGGGCTTCACTGATGGTAG‐3′	NM_008318
ALP	Forward: 5′‐GGCTCTGCCGTTGTTTCTCT‐3′ Reverse: 5′‐AAGGTGCTTTGGGAATCTGC‐3′	NM_007431
ColⅠ	Forward: 5′‐GGTCCTTCTGGTCCTCGTG‐3′ Reverse: 5′‐TCTCCGTTCTTGCCAGGA‐3′	NM_007732
Runx2	Forward: 5′‐CTGCCACCTCTGACTTCTGC‐3′ Reverse: 5′‐GATGAAATGCCTGGGAACTG‐3′	NM_001146038
GAPDH	Forward: 5′‐ACAGCAACAGGGTGGTGGAC‐3′ Reverse: 5′‐TTTGAGGGTGCAGCGAACTT‐3′	NM_008084