Figure 2.

Overexpression of miR‐502‐5p inhibits the proliferation and migration of BCa cells. A, CCK8 assay. The relative cell viabilities of T24 and UM‐UC3 after exposure to different concentrations of the miR‐502‐5p mimic were notably lower than those in the NC‐treated groups at 48 and 72 h. B, Colony formation assay (representative wells are presented). The colony formation rate was lower for the miR‐502‐5p mimic‐treated group than for the NC‐treated group. C, Cell cycle assay (representative histograms are presented). Overexpression of miR‐502‐5p notably induced G1 phase arrest in T24 and UM‐UC3 cell lines. D, Wound‐healing assay. The wound‐healing efficacy of the miR‐502‐5p mimic (50 nmol/L)‐transfected group was retarded at 24 h in the T24 cell line. E, Transwell assay (representative micrographs are presented). Migration rates were reduced by the overexpression of miR‐502‐5p. F, Cell apoptosis assay (representative histograms are presented). Overexpression of miR‐502‐5p significantly induced cell apoptosis in the T24 and UM‐UC3 cell lines. G, Western blot assay. Noticeable inhibition of EMT, MMPs, cell apoptosis and cell cycle‐associated proteins were detected by the overexpression of miR‐502‐5p in BCa cells. Error bars represent the SE obtained from 3 independent experiments; *P < .05