Figure 5.

Silencing of DNMT3B inhibits the cell migration and proliferation of T24 and UM‐UC3 cell lines. A, CCK8 assay. Silencing of DNMT3B significantly inhibited cell viability. B, Colony formation assay (representative wells are presented). The colony rate of the si‐DNMT3B transfected group was significantly reduced compared with the NC‐treated group. C and D, Cell cycle assay (representative histograms are presented). Treatment of the siRNA pool in DNMT3B markedly induced G1 phase arrest both in T24 and UM‐UC3 cell lines. E, Western blot assay. Three DNMT3B siRNAs were merged into the siRNA pool to obtain higher interference efficiency. F, Western blot assay. Si‐DNMT3B inhibited EMT and cell cycle‐related proteins. G, Wound‐healing assay. Silencing of DNMT3B retarded the healing of T24 cells at 24 h. H, Transwell assay (representative micrographs are presented). si‐DNMT3B impaired migration of T24 and UM‐UC3 cells. I, Schematic diagram showed the promoter region of E‐cadherin. J, Methylation status of each CpG island detected and calculated by bisulphite‐sequencing PCR experiment. And percentage of methylated CpG islands was performed. K, The expression of miR‐502‐5p was upregulated after silencing DNMT3B. Error bars represent the SE obtained from three independent experiments; *P < .05; **P < .01; ***P < .001