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. 2020 Feb 17;58(1):176–183. doi: 10.1080/13880209.2020.1723648

Figure 4.

Figure 4.

Effects of DCAE-rich medium on cardiomyocytes hypertrophy induced by isoproterenol in vitro. (A) The effects of different concentrations of DCAE on the activity of H9c2 cells at different times (n = 6). (B) The relative mRNA levels of hypertrophic marker ANP to GAPDH (n = 3). (C) The relative mRNA levels of hypertrophic marker BNP to GAPDH (n = 3). (D) H9c2 cell phalloidin staining (scale bar 100 μm). (E) Summary graph of H9c2 cells area (n = 3). Each value shown represents mean ± SEM. *p < 0.05, **p < 0.01 vs. the control group. #p < 0.05, ##p < 0.01 vs. the isoproterenol group.