FIG 5.

hnRNP K supports high-amplitude Dbp oscillation and Dbp-dependent clock genes such as Period2, Rev-erbα, and RORβ. (A) Dbp, Cry1, eif4e, Rev-erbα, Per2, and RORβ mRNA oscillations were measured after dexamethasone synchronization. CircWave, which is a program that is able to generate sine waves based on its algorithm, showed mRNA oscillation of each clock gene. The hnRNP K knockdown group showed a decrease in mRNA amplitude of Dbp and Dbp-dependent clock genes (n = 3 or 4; n.s., not significant). Error bars indicate SEM). sihnK, sihnRNP K. (B) Our proposed model of the regulation by hnRNP K, which leads to the regulation of Dbp and Dbp-dependent genes such as Rev-erbα, Per2, and RORβ. **, poly(C) motif.